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. 2021 May 5;4(2):245–259. doi: 10.1002/rco2.41

Figure 5.

Figure 5

Caffeine contracture force of adult and old WT and Draggen soleus. (A) Caffeine contracture force compared for WT and Draggen mice at two ages: adult (13–42 weeks) and old (91–103 weeks). Individual data points are shown. (B) The ratio of caffeine contracture to tetanic force is compared for WT and Draggen mice at the same two ages as (A). Individual data points are shown. (C) Ponceau to compare total protein and Western blot to measure RyR1 expression in adult and old Draggen soleus (Abcam 2868 1:1000) AD = adult Draggen (18–68 weeks), OD = old Draggen (99–100 weeks); ****P < 0.001.