Figure 2.

Establishment of MSC senescence model by H₂O₂ stimulation. (a) Sa-β-gal-positive cells were significantly increased under H₂O₂ treatment. (b) H₂O₂ treatment reduced cell proliferation of MSCs. (c) The effect of H₂O₂ treatment on cell cycle phase by Flow cytometry analysis. (d) H₂O₂ (100 μmol/L) only leaded slight MSCs apoptosis. (e) The expression of P21 and P16 was increased under H₂O₂ treatment. Data are expressed as mean ± SD (n = 5). The asterisk indicates a significant difference (p < 0.05).