Figure 7.

YAP knockdown reduced Gefitinib resistance and suppresed cell malignant behaviors in PC9/GR and HCC827/GR cells by upregulating PD-L1. (a) The expression of PD-L1 protein in PC9/GR, HCC827/GR, and their parental cells was detected by Western blot. (b) The efficiency of sh-YAP was checked by Western blot. In PC9/GR and HCC827/GR cells transfected with sh-YAP or sh-YAP+oe-PD-L1, (c) the expression of PD-L1 protein was detected by Western blot. (d and e) IC50 of Gefitinib was concluded by CCK-8 assay. (f) Cell proliferation was assessed by colony formation assay. (g) Cell proliferation was also assessed by EdU assay. (h) Cell cycle progression was determined by flow cytometry assay. (i) Cell apoptosis was determined by flow cytometry assay. (j) The protein levels of CDK4, Bax and cleaved-caspase3 were measured by Western blot. *P < 0.05.