Figure 2.

PFKFB3 regulates chemoresistance in ovarian cancer. (A) Protein levels of PFKFB3 in A2780S and A2780CP cells treated with cisplatin (0, 2.5, 5 and 10 µM) for 48 h, determined by immunoblotting. (B) Relative lactate production level of A2780CP cells treated with 3PO (0, 10 µM), determined by Lactate Colorimetric Assay Kit II. (C) A2780CP cells treated with 3PO (0 and 10 µM) and cisplatin (0 and 20 µM) for 48 h, cell apoptosis was detected by Annexin V- FLUOS staining. (D) Protein expression of PARP, cleaved PARP, c-IAP1, c-IAP2 and survivin in A2780CP cells treated with 3PO (0 and 10 µM) and cisplatin (0 and 20 µM) for 48 h, determined by immunoblotting. (E) Bar chart of BIRC2, BIRC3, BIRC5 and CD70 genes mRNA expression in A2780CP cells treated with 3PO (0 and 10 µM) and cisplatin (0 and 20 µM) for 48 h, determined by the human apoptosis RT² profiler PCR array. (n = 3; *p < 0.05; **p < 0.01).