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. 2022 Jan 28;12:748403. doi: 10.3389/fonc.2022.748403

Figure 5.

Figure 5

PFK158 suppresses stemness in ovarian cancer. (A) Transwell migration and invasion (scale bar, 100 μM) and (B) sphere formation (scale bar, 100 μM) of ALDH+ CD44+ SKOV3 cells pretreated with control and PFK158 (0.5 and 1.0 μM) for 48 h. (C) Relative KLF4 mRNA expression of ALDH+CD44+ SKOV3 cells treated with 1.0 μM PFK158, determined by qPCR. (n=3; *p < 0.05, **p < 0.01). (D) After inoculation of 3×105 ALDH+CD44+ SKOV3 cells pretreated with control (n = 5) and PFK158 (1.0μM, n = 5) into the right/left flanks of mice, images of dissected tumors and tumor formation days were recorded. (E) Line chart showing the tumor growth and bar chart showing the weights of tumors derived from the mice injected with 3×105 control and PFK158-pretreated ALDH+CD44+ SKOV3 cells. (F) Tumor incidence and estimated frequency from in vivo limiting dilution assays with injection of a limiting dilution (3×105, 10×103, 5×103, or 2.5×103 cells) of control and PFK158-pretreated ALDH+CD44+ SKOV3 cells into the flanks of mice.