Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 12;3:100021. doi: 10.1016/j.crviro.2022.100021

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Published by Elsevier B.V.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

PMC Copyright notice

Fig. 3 — TLR expression in NSP1 and NSP15 stably expressing A549 cells. Representative overlapping FACS histogram demonstrating the expression of (A) TLR2 (B) TLR3 (C) TLR4 and (D) TLR9 gated on live (7AAD-) cells to analyze (E) the number of live cells expressing TLR2, TLR3, TLR4 and TLR9 in NSP1 and NSP15 stably expressing A549 cells lines compared to negative control (A549 cells). (F) Fold changes in gene (Interferon regulatory factor 1; IRF1 and IRF7) expression based on qRT-PCR in NSP1 and NSP15 stably expressing A549 cells lines over negative control (A549 cells). Non-parametric Wilcoxon tests (Mann-Whitney) was used to assess normal distribution and test significance with the results shown as mean ± SD. ∗ (p ≤ 0.05), and ∗∗∗ (p ≤ 0.005) indicates a statistically significant difference. All experiments were performed in 3 technical replicates, and the data are representative of results from minimum of 3 independent experiments.