Table 1.
Target | Primer sequencesa | Accession No. | |
---|---|---|---|
NSP1 1/3 | Fwd | AAGCTTGCCACCATGGAGAGCCTTGTCC | NC_045512.2 |
Rev | GATATCCAAGTTGAGGCAAAACGCCT | ||
NSP1 2/3 | Fwd | AAGCTTGCCACCTGCCTCAACTTGAACAGCCC | NC_045512.2 |
Rev | GATATCCTTGCGGTAAGCCACTGGTA | ||
NSP15 1/3 | Fwd | AAGCTTGCCACCATGGTTTAGAAAATGTGGCT | NC_045512.2 |
Rev | GATATCTCGTTTCAGTTGGTTTCTTGGC | ||
NSP15 2/3 | Fwd | AAGCTTGCCACCTTGTGCACCACTCACTGTCT | NC_045512.2 |
Rev | GATATCCAATTTCCATTTGACTCCTGGGT |
The boldface at 5' ends represents the HindIII and EcoRV restriction sequences inserted for restriction cloning into the pCDNA3.1/V5–HIS-TOPO plasmid.