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. 2022 Feb 12;3:100021. doi: 10.1016/j.crviro.2022.100021

Table 1.

Primer sequences used for PCR.

Target Primer sequencesa Accession No.
NSP1 1/3 Fwd AAGCTTGCCACCATGGAGAGCCTTGTCC NC_045512.2
Rev GATATCCAAGTTGAGGCAAAACGCCT
NSP1 2/3 Fwd AAGCTTGCCACCTGCCTCAACTTGAACAGCCC NC_045512.2
Rev GATATCCTTGCGGTAAGCCACTGGTA
NSP15 1/3 Fwd AAGCTTGCCACCATGGTTTAGAAAATGTGGCT NC_045512.2
Rev GATATCTCGTTTCAGTTGGTTTCTTGGC
NSP15 2/3 Fwd AAGCTTGCCACCTTGTGCACCACTCACTGTCT NC_045512.2
Rev GATATCCAATTTCCATTTGACTCCTGGGT
a

The boldface at 5' ends represents the HindIII and EcoRV restriction sequences ​inserted for restriction cloning into the pCDNA3.1/V5–HIS-TOPO plasmid.