Table 2.

Primer sequences used for Real-Time PCR.

Target	Primer sequence		Accession No.	Annealing temp (oC)
Pan-IFNα	Fwd	CACACAGGCTTCCAGGCATTC	NC_045512.2	61
	Rev	TCTTCAGCACAAAGGACTCATCTG
IFN-β	Fwd	TGCTCTCCTGTTGTGCTTCTCCAC	NM_002176.4	61
	Rev	ATAGATGGTCAATGCGGCGTCC
IFN-λ1	Fwd	CGCCTTGGAAGAGTCACTCA	NM_172140.2	61
	Rev	GAAGCCTCAGGTCCCAATTC
IRF-1	Fwd	TCTTAGCATCTCGGCTGGACTTC	NM_002198.3	61
	Rev	CGATACAAAGCAGGGGAAAAGG
IRF-7	Fwd	GATGTCGTCATAGAGGCT GTTGG	NM_004029.4	61
	Rev	TGGTCCTGGTGAAGCTGGAA
ISG15	Fwd	GAGAGGCAGCGAACTCATCT	NM_005101.4	60
	Rev	AGGGACACCTGGAATTCGTT
ISG54	Fwd	ATTCTATCACCAAGCCCGTGG	NM_001547.5	60
	Rev	TGGAGTCTGGAAGCCTCATCC
ISG56	Fwd	CAGCAACCATGAGTACAAAT	NM_001548	60
	Rev	AAGTGACATCTCAATTGCTC
OAS1	Fwd	GCCCTGGGTCAGTTGACTGG	NM_016816	60
	Rev	TGAAGCAGGTGGAGAACTCGC
PKR	Fwd	ACACTCGTCTCTGAATCATC	NM_002759	60
	Rev	GAGACCATTCATAAGCAACG
IFIT1	Fwd	GATCTCAGAGGAGCCTGGCTAA	NM_001548.5	60
	Rev	TGATCATCACCATTTGTACTCATGG
IFITM3	Fwd	GATGTGGATCACGGTGGAC	NM_021034.3	61
	Rev	AGATGCTCAAGGAGGAGCAC
VIPERIN	Fwd	GAGAGCCATTTCTTCAAGACC	NM_080657	60
	Rev	CTATAATCCCTACACCACCTCC
GAPDH	Fwd	TACTCCTTGGAGGCCATGTG	NM_001357943.2	61
	Rev	CACAGTCCATGCCATCACTG