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. 2022 Feb 12;14:100116. doi: 10.1016/j.medntd.2022.100116

Table 1.

Summary of the characteristics of some NAT methods and immunoassay-based methods.

Detection Method Timing Sensitivity Cost Advantages Disadvantages
Nucleic-acid Testing Methods RT-qPCR 2–3 ​h High High Wide detection range; High throughput Relatively higher cost; False-negative results
RT-LAMP 0.5–1 ​h High Medium Easy opreation; Isothermal Amplification False-positive results
RPA 10–20 ​min High High Ultra-fast speed; Isothermal amplification (37 ​°C) Lower flexibility in the kit formulation
NEAR 15–30 ​min High Low Fast amplification speed; Isothermal amplification False-positive results with short primers
RCA 4 ​h High Low Simple reaction mechanism; Isothermal amplification; Complex sample preparation;
CRISPR-based 0.5–1 ​h High High High efficiency, High Specificity, and precision Potential aerosol contamination
Immunoassay-based Methods LFI 10 ​min Low Low Easy opreation and detection; Low cost Qualitative dtection; False-positive
Chemliuminescence 0.5–1 ​h Medium Low High-throughput; Good accuracy Multiple opeation steps, Higher instrumentation requirements
Electrochemistry 10–30 ​min Medium Low Fast quantitative detection High requirements for reaction conditions
FET 10–30 ​min Medium Low Fast quantitativedetection; Easy operation and integration High requirements for reaction conditions