Nucleic-acid Testing Methods |
RT-qPCR |
2–3 h |
High |
High |
Wide detection range; High throughput |
Relatively higher cost; False-negative results |
RT-LAMP |
0.5–1 h |
High |
Medium |
Easy opreation; Isothermal Amplification |
False-positive results |
RPA |
10–20 min |
High |
High |
Ultra-fast speed; Isothermal amplification (37 °C) |
Lower flexibility in the kit formulation |
NEAR |
15–30 min |
High |
Low |
Fast amplification speed; Isothermal amplification |
False-positive results with short primers |
RCA |
4 h |
High |
Low |
Simple reaction mechanism; Isothermal amplification; |
Complex sample preparation; |
CRISPR-based |
0.5–1 h |
High |
High |
High efficiency, High Specificity, and precision |
Potential aerosol contamination |
Immunoassay-based Methods |
LFI |
10 min |
Low |
Low |
Easy opreation and detection; Low cost |
Qualitative dtection; False-positive |
Chemliuminescence |
0.5–1 h |
Medium |
Low |
High-throughput; Good accuracy |
Multiple opeation steps, Higher instrumentation requirements |
Electrochemistry |
10–30 min |
Medium |
Low |
Fast quantitative detection |
High requirements for reaction conditions |
FET |
10–30 min |
Medium |
Low |
Fast quantitativedetection; Easy operation and integration |
High requirements for reaction conditions |