Skip to main content
. 2021 Oct 19;41(7):943–959. doi: 10.1038/s41388-021-02060-5

Fig. 3. The AKT inhibitor (AKTi) reduces the androgen-induced cellular senescence in both CSPC and CRPC cells.

Fig. 3

Fig. 3

LNCaP cells were treated for 72 h with SAL, LAL, or 0.1% DMSO as solvent control. A The level of p-AKT is enhanced in both LNCaP and C4-2 cells treated for 72 h with SAL (n = 3). B Induction of p-AKT levels in CDKN2B-knockdown cells. Numbers indicate mean ± SD. C Detection of SA-β-Gal activity of LNCaP and C4-2 cells incubated for 72 h with DMSO, LAL, or SAL in combination with or without AKT inhibitor (AKTi). Bars show means ± SD (n = 3). D Analyses of mRNA level of CDKN2B by the indicated treatments in LNCaP and C4-2 cells. Expression was normalized to the housekeeping genes TBP and GAPDH (n = 3). E Detection of p15 protein level. F Detection of p-AKT and its downstream target p-S6 (n = 3). **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.