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. 2021 Dec 27;39(1):89–103. doi: 10.1007/s11095-021-03158-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — The cytotoxicity of ADC aggregates. (a) The cytotoxicity of aggregated ADCs in HER2-positive cells. (b) The cytotoxicity of aggregated ADCs in HER2-negative cells. The HER2-positive cells (SK-BR-3) or HER2-negative cells (Jurkat, MEG-01S, THP-1, TMNK-1) were incubated with serially diluted samples (control, aggregates, or filtrated samples) for 3 days, and the cell proliferations were measured by WST-8 assay. The concentrations of filtered samples represent pre-filtration concentration of the samples. The data represent individual plots (n = 2).