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. 2021 Dec 27;39(1):89–103. doi: 10.1007/s11095-021-03158-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Influence of FcγRIIa-blocking on the cytotoxicity of ADC aggregates in MEG01-S cells. FcγRIIa on MEG01-S cells were blocked by using a Fab fragment of anti-FcγRIIa antibody (Fab-IV.3). MEG01-S cells were pre-treated with Fab-IV.3 (final concentration: 2 μg/mL) for 30 min, and then the serially diluted samples were added to the FcγRIIa-blocking MEG01-S cells. (a) The cellular uptake of Tra-FL aggregates under an FcγRIIa-blocking condition. The control cells, FcγRIIa-blocking cells, and cells under a low temperature condition were incubated with the serially diluted samples (control or aggregates; final concentration: 5, 13, 32, or 80 nM) for 4 h. The fluorescent intensities were measured by flow cytometer. The data represent the means ± standard deviation (n = 3). (b) The cytotoxicity of ADC aggregates under an FcγRIIa-blocking condition. The control cells and FcγRIIa-blocking cells were incubated with serially diluted samples (control, aggregates, or filtrated samples) for 3 days, and the cell proliferations were measured by wst-8 assay. The concentrations of filtered samples represent pre-filtration concentration of the samples. The data represent individual plots (n = 2).