Table 2.

A survey of published protocols for intact muscle preparation, culture and testing. This list focuses on unique downstream applications and methods and is not comprehensive. FDB: flexor digitorum brevis, EDL: extensor digitorum longus.

Reference	Muscle	Applications	Features
Wilkinson et al. [84]	EDL	Muscle-nerve co-culture, electrical stimulation, passive stretching, recording of muscle spindle afferrents	Describes recording sensory information from attached nerve
Kerschensteiner et al. [58]	Triangularis Sterni	Muscle-nerve co-culture, timelapse microscopy, fixation and immunostaining	Details neuronal degeneration in culture
Hanson et al. [46]	Diaphragm	Muscle-xenonerve co-culture, electrical stimulation, drug treatment, fixation and immunostaining	Describes xenonerve culture
Clark et al. [85]	Lumbrical	Muscle-nerve co-culture, neurotoxin delivery, electrical stimulation, fixation and immunostaining	Protocol for isolating multiple preparations from a single mouse
Hansen et al. [86]	Epitrochlearis, FDB & Soleus	Electrical stimulation, insulin stimulation, glucose uptake, hexokinase activity	An in-vitro 2-deoxyglucose assay for muscle
Park et al. [55]	EDL & Soleus	Electrical stimulation, force-frequency relationship, fatigue, mechanic alternans	Video demonstration of muscle dissection and mounting
Moorwood et al. [51]	EDL and Diaphragm strips	Electrical stimulation, eccentric contraction testing	Video demonstration of muscle dissection and mounting