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. 2022 Feb 11;12:2322. doi: 10.1038/s41598-022-05857-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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sGRP78 expression on bone marrow cells of pediatric individuals with newly diagnosed B-ALL. (A) Acute lymphocytic leukemia cell lines were stained with anti-GRP78 antibody and representative experiments of each cell line are depicted. (B) Statistical representation of cell surface GRP78⁺ cells in leukemic cells lines is shown. Data are the mean plus SEM of n = 3. Statistical analysis was performed by One-way ANOVA, p < 0.0001. (C) Gating strategy for sGRP78⁺ cells analysis from bone marrow derived cells of pediatric individuals with B-ALL and controls (BM-CTL). (D) Statistical representation of cell surface GRP78⁺ cells in BM-CTL (n = 4) and B-ALL (n = 43). Statistical analysis was performed by two-tailed Mann–Whitney U test, *p < 0.05, **p < 0.01.