Fig. 3. Arabidopsis hypocotyl elongation complementation assays with PsKAI2s.

Hypocotyl length of 7-day-old seedlings grown under low light at 21 °C. Data are means ± SE (n = 20–24; 2 plates of 10-12 seedlings per plate). Light blue bars: Mock (DMSO), lavender bars: (–)-GR24 (1 µM). Complementation assays using the AtKAI2 promoter to express AtKAI2 (control) or PsKAI2 genes in the htl-3 mutant background (Col-0 ecotype) as noted above the graph. Proteins were tagged with mCitrine or GUS protein. For DMSO controls, statistical differences were determined using a one-way ANOVA with a Tukey multiple comparison of means post-hoc test, statistical differences of p < 0.05 are represented by different letters. Means with asterisks indicate significant inhibition compared to mock-treated seedlings with *** corresponding to p ≤ 0.0001, ** corresponding to ≤0.001 and * to p ≤ 0.01, as measured by t test.