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. 2022 Feb 11;12:2390. doi: 10.1038/s41598-022-06114-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of LPIN1 overexpression in the BMECs. (A) Fluorescence results of LPIN1 overexpression in the BMECs. (A1). Green fluorescence of control group (pEGFP-C1); (A2) Ordinary light of control group (pEGFP-C1); A3. Green fluorescence of pEGFP-C1-LPIN1; A4. Ordinary light of pEGFP-C1-LPIN1; (B) The efficiency of LPIN1 overexpression; (C) The overexpression of LPIN1 up-regulates the expression of genes related to milk fat synthesis in the BMECs; (D) Changes of total TAG content in the BMECs under the LPIN1 overexpression. Values are displayed as means ± SEM for three biological replicates. *, P < 0.05; **, P < 0.01.