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. 2022 Feb 11;12:2390. doi: 10.1038/s41598-022-06114-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The effects of PPARG overexpression on the LPIN1 mRNA expression and promoter activity in the BMECs. (A) The mRNA expression of PPARG after transfection with pEGFP-C1-PPARG; (B) The mRNA expression of LPIN1 after transfection with pEGFP-C1-PPARG; (C) Relative luciferase activity (Firefly: Renilla) after 48 h transfection of pEGFP-C1-PPARG and different 5’ flanking deletion constructs (− 1878 bp/ + 42 bp, − 666/ + 42 bp, − 416/ + 42 bp, − 336/ + 42 bp, − 172/ + 42 bp and − 88/ + 42 bp. Values are provided as mean ± SEM; *P < 0.05; **P < 0.01.