Table 2.
The information of RT-qPCR primer sets used in this study.
| Gene | Accession number | Primer sequences(5′-3′) | Product length (bp) | Amplification efficiency |
|---|---|---|---|---|
| YWHAZ | XM_006046432 | F: GAAAGGGATTGTGGATCAG | 184 | 2.012 |
| R: GGCTTCATCAAATGCTGTCT | ||||
| GAPDH | XM_006065800 | F: ATGGAGAAGGCTGGGGCTCA | 144 | 2.058 |
| R: GCAGGAGGCATTGCTGACAA | ||||
| ACTB | NM_001290932 | F: TGGGCATGGAATCCTG | 196 | 2.032 |
| R: GGCGCGATGATCTTGAT | ||||
| R: ACCCTGGTCACAAACTGAATGCT | ||||
| GPAM | XM_006043939 | F: ACTACGGATGTGTCAGAGTGGAT | 141 | 1.986 |
| R:CACTGGGTCTTGAGGGAAGTATAG | ||||
| DGAT2 | XM_006045187 | F: GTCCTGTCTTTCCTCGTGCT | 141 | 2.031 |
| R: CCTCCTGCCACCTTTCTT | ||||
| AGPAT6 | NM_001290846 | F:CTTTGCGTGGGCTACCTTG | 242 | 2.0.32 |
| R: TCTTGGTCACCTCGTCGTC | ||||
| BTN1A1 | NM_174508 | F: GACTATCTGCCCAATCGCTGAT | 153 | 2.116 |
| R: GGGCTGGAGAGGGATTAGTTTA | ||||
| LPIN1 | XM_006073743 | F: CAGTCGAGGCTCAGACCA | 232 | 2.086 |
| R: TTCCCCGTTGATTTCTATGTCA | ||||
| PPARG | XM_006077448 | F: GCTCCAAGAGTACCAAAGTG | 204 | 2.096 |
| R: GTCCTCCTGAAGAAACCCTT | ||||
| SREBF1 | KU517672 | F: GCACCGAGGCCAAGTTGAATAA | 146 | 2.041 |
| R: CAGGTCCTTCAGCGATTTGCTT | ||||
| SREBF2 | XM_006068914 | F:GCCAAGATGCACAAGTCTGGTGTT | 136 | 2.099 |
| R: TGCCCTTCAGGAGCTTGCTCT | ||||
| DGAT1 | NM_001290902 | F: ACAGACAAGGACGGAGACG | 268 | 1.988 |
| R: CCACAATGACCAGGCACA |