Table 3.

Primers used in the construction of luciferase reporter vector.

Purpose	Primer ID	Primer sequences	Binding region (bp)	Anneal temperature/°C
Cloning	LPIN1_F	CTCGAGCCTAGGAGCCCAAGTCAGGT	− 1878	61.5
	LPIN1_R	CCCAAGCTTGCTGCCTTTGTATGCAAACACCC	+ 42	61.5
5’ deletion	F-1523	CCGCTCGAGTCAGACAGTGGAGGAGTCGTAA	− 1523	59.2
	F-1264	CCGCTCGAGATACTGGCGTGGGTAGCCGTTCC	− 1264	61.2
	F-953	CCGCTCGAGGTTAGTGACAGGGCTTGGAGTAT	− 953	59.4
	F-666	CCGCTCGAGCTGGGTGATTGCAGACCA	− 666	61.4
	F-416	CCGCTCGAGCTTCGCCAACGAGCACCAGA	− 416	62.9
	F-336	CCGCTCGAGACGGGCACTCTTGCTTCCTT	− 336	62.9
	F-172	CCGCTCGAGGGCTCACTCCTCGAAAGGGC	− 172	64.7
	F-88	CCGCTCGAGCTCTCGCACCACCCCGCTG	− 88	65
	R + 42	CCCAAGCTTGCTGCCTTTGTATGCAAACACCC	+ 42	61.5
Site-directed mutation	PPRE1	TGGCCTTGTGACAG		62
	PPRE2	GGGTCGAAAGATCT		62
	PPRE1-mut	CCCTGCAAAAGTCTGGAGTTGACACAGTAATAGGGAGAGG		59.2
	PPRE1-anti-mut	CCTCTCCCTATTACTGTGTCAACTCCAGACTTTTGCAGGG		61.2
	PPRE2-mut	GCCCCGGGGGCGGTATCGAAAGAGTTCTCGCACCAC		59.4
	PPRE2-anti-mut	GTGGTGCGAGAACTCTTTCGATACCGCCCCCGGGGC		61.4

Plus ( +) and minus (-) indicate the number of nucleotides upstream (−) and downstream ( +) from the transcriptional start site. The underlined bases are the restriction site. The bases of italic are the protective bases. Mutation bases are shown in bold font.