Skip to main content
. 2022 Feb 11;8:15. doi: 10.1038/s41531-022-00278-y

Fig. 2. Rescue of neuropathological features in patient-derived p.A53T neurons by BX795.

Fig. 2

a BX795 has a positive effect on neurite length of p.A53T-neurons. Representative confocal images of healthy control (ctl) and p.A53T-neurons immunostained for TH and quantification of total neurite length of TH+ cells. Data represent mean ± SEM. (Comparisons by ANOVA with Tukey correction *P < 0.05, **P < 0.01, n = 4 independent experiments with at least 50 cells analyzed in each experiment). Scale bar, 50 μm. b BX795 alleviates axonal neuropathology in p.A53T-neurons. Higher magnification at the right (upper, DMSO-treated cells; lower, BX795-treated cells) shows neurites with swollen varicosities or fragmented processes (arrows). Scale bar, 30 μm. Quantification of axonal degeneration is estimated in the accompanying graph by measuring the ratio of TUJ1+ spots over the total TUJ1+ area in untreated (p.A53T) or BX795-treated p.A53T-neurons. Data represent mean ± SEM.(Comparisons by ANOVA with Tukey correction, *P < 0.05, **P < 0.01, n = 20 randomly selected fields for each condition; data was from three independent experiments). c BX795 reduces protein aggregates in p.A53T-neurons. Representative confocal images showing protein aggregates in p.A53T TUJ1+ neurons (Scale bar, 10μm) and quantification in untreated or BX795-treated TUJ1+ cells (Data was from three independent experiments. Mann–Whitney test; n = at least 30 cells per group; ****P < 0.0001). d Detection and quantification of p(Ser129)αSyn by Western blot in control and p.A53T neurons in the absence or presence of BX795, as indicated; Actin shows equal protein loading. Data represent mean ± SEM (t test, *P < 0.05, n = 4 independent experiments).