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. 2022 Jan 21;27(3):708. doi: 10.3390/molecules27030708

Table 3.

Crystallization conditions and structural data of the reported IspH.

PDB ID Release Date Source Organism Crystallization Method
(Vapor difusion)
Crystal Growth Procedure pH Space Group Res. (Å) Cluster Ligand ID Ref
3DNF 30 December 2008 A. aeolicus Hanging drop 0.1 M Tris-Hcl, 10% PEG 8000 8.0 P 1 21 1 1.65 Fe3 S4 - [36]
4N7B 20 November 2013 P. falciparum Sitting drop 0.1 M Na citrate, 0.1 M (NH4)2SO4, 30% PEG 4000 5.6 P 31 2 1 2.20 Fe3 S4 SO4 [43]
3F7T 7 July 2009 E. coli Hanging drop 1.6 M Potassium Phosphate 8.0 P 32 2 1 1.80 Fe3 S4 POP [42]
3KEF 12 January 2010 E. coli Sitting drop 0.1 M Bis-Tris, 0.2 M Li2SO4, 25% PEG 3350 6.5 P 21 21 21 1.70 Fe3 S4 DMA [37]
3KEL 1.80 Fe3 S4 POP
3KE8 1.70 Fe4 S4 EIP
3KE9 1.90 Fe4 S4 IPE
3KEM 7.0 C 1 2 1 2.00 Fe3 S4 IPE
3T0G 30 November 2011 E. coli Hanging drop 0.1 M Bis-Tris, 0.2 M Li2SO4, 25% PEG3350 6.5 P 21 21 21 2.10 Fe3 S4 H6P [45]
3T0F 1.90 Fe3 S4 H6P
3SZU 1.40 Fe3 S4 H6P
3SZL 1.60 Fe4 S4 H6P
3SZO 1.60 Fe4 S4 H6P
3URK 5 September 2012 E. coli Sitting drop 0.1 M Bis-Tris, 0.2 M (NH4)2SO4, 25% PEG3350 6.5 P 21 21 21 1.50 Fe4 S4 0CG [46]
3UTC 1.90 Fe4 S4 0JX
3UTD 1.70 Fe3 S4 OCJ
3UV3 1.60 Fe4 S4 0CM
3UV6 1.70 Fe4 S4 0CH
3UV7 1.60 Fe4 S4 0CN
3UWM 1.80 Fe4 S4 0K2/0JX
3ZGL 9 January 2013 E. coli Hanging drop 0.1M Bis-Tris, 0.2 M Li2SO4, 24% PEG 3350 6.5 C 1 2 1 1.68 Fe4 S4 10E [47]
3ZGN 1.95 Fe4 S4 10G
4H4C 23 January 2013 E. coli Sitting drop 0.1 M Bis-Tris, 0.2 M (NH4)2SO4, 25% PEG3350 6.5 P 21 21 21 1.80 Fe4 S4 10D [48]
4H4D 1.35 Fe4 S4 10E
4H4E 1.70 Fe4 S4 10G
4EB3 6 February 2013 E. coli Sitting drop 0.1 M Bis-Tris, 0.2 M (NH4)2SO4, 25% PEG3350 6.5 P 21 21 21 1.90 Fe4 S4 0O3 [49]
4MUY 11 June 2014 E. coli Sitting drop 0.1 M Bis-Tris, 0.2 M (NH4)2SO4, 25% PEG3350 6.5 P 21 21 21 1.80 Fe3 S4 2E5 [50]
4MV0 1.90 Fe3 S4 2E6
4MV5 18 June 2014 1.90 Fe3 S4 2E7
4MUX 1.70 Fe4 S4 2E4