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. 2022 Feb 4;27(3):1066. doi: 10.3390/molecules27031066

Figure 6.

Figure 6

Transient transfection of AQP4 regulated MMP-9 and TRAF6 expression. Neuro-2a cells were transiently transfected with pcDNA3.0-AQP4 for 48 h. After transfection for 24 h, 10 μM of ACR was added for the remaining 24 h. Transient transfection of AQP4 inhibited MMP-9, TRAF6, and NFκB expression but did not affect nNOS or nAChR expression (A). In the ACR-treated groups, ACR inhibited AQP4 expression and inhibited TRAF6 and TNF-α expression. The quantitative data showing AQP4 (B), MMP-9 (C), TRAF6 (D), TNF-α (E), NFκB (F), nNOS (G), and nAChR (H) protein levels. *: Significant difference versus the control group, #: Significant difference versus the ACR treatment group.