Table 2.
Samples for total RNA sequencing of Hass tissues and corresponding quality statistics. NanoDrop values and Bioanalyzer results indicate the quality of the extracted RNA. An RIN (RNA integrity number) above 6 is considered acceptable quality RNA; here in most cases, an RIN value above 6 and a very high concentration of total RNA were obtained. The concentration was calculated relative to an Agilent RNA 6000 Nano Ladder using a Bioanalyzer 2100 instrument (Agilent Technologies, Santa Clara, California, USA). Details of tissues are reported in Supplementary Material Table S1.
| Tissue | 260/280 | 260/230 | RIN | Concentration (ng/μL) | Total Amount * (μg) |
|---|---|---|---|---|---|
| Embryo | 2.08 | 1.83 | 8.6 | 579 | 11.58 |
| Seedling | 2.06 | 1 | 8.4 | 306 | 6.12 |
| Callus | 1.89 | 1.15 | 8 | 192 | 9.6 |
| Feeder root | 1.37 | 1.05 | 6.5 | 704 | 35.2 |
| Pedicel | 1.89 | 0.69 | 7.1 | 825 | 16.5 |
| Leaves | 2.08 | 1.73 | 6.4 | 2744 | 137.2 |
| Woody stem | 1.81 | 0.57 | 6.9 | 876 | 17.52 |
| Young stem | 1.93 | 0.9 | 6.4 | 1362 | 27.24 |
| Male flower | 1.97 | 0.93 | 8.5 | 1230 | 24.6 |
| Female flower | 2.03 | 1.15 | 7.7 | 2769 | 55.38 |
| Flower bud | 2.01 | 1.16 | 7.5 | 2097 | 41.94 |
| Unripe fruit pulp and peel | 2.16 | 1.23 | 6.8 | 576 | 28.8 |
| Ripe fruit pulp and peel | 2.1 | 1.74 | 5.9 | 2096 | 104.8 |
| Immature fruit | 2.01 | 2.17 | 7.3 | 5665 | 283.25 |
| Unripe fruit seed | 2.09 | 1.29 | 6 | 640 | 32 |
| Ripe fruit seed | 1.71 | 0.72 | 5.7 | 552 | 27.6 |
| Seedling leaf | 2.21 | 2.4 | 6.6 | 2568 | 128.4 |
| Lateral root | 1.87 | 0.92 | 7.8 | 112 | 5.6 |
| Shoot | 2.17 | 1.86 | 7.3 | 1028 | 51.4 |
| Tap root | 2.18 | 2.08 | 7.3 | 1408 | 70.4 |
* Total RNA extracted was from 0.01 to 0.03 g of frozen ground tissue.