TABLE 2.

Primers of virF, YPMs, and HPI genes

Virulence factor	Target	Orientationa	Sequence (5′–3′)	Size of product (bp)	Annealing temp. (°C)	Reference
pYV	virF	SP	TCATGGCAGAACAGCAGTCAG	590	55	48
		ASP	ACTCATCTTACCATTAAGAAG
YPMa	ypmA	SP	CACTTTTCTCTGGAGTAGCG	350	55	27
		ASP	GATGTTTCAGAGCTATTGTT
YPMb	ypmB	SP	TTTCTGTCATTACTGACATTA	453	52	36
		ASP	CCTCTTTCCATCCATCTCTTA
YPMc (for sequence)	ypmA and ypmC	SP	ACACTTTTCTCTGGAGTAGCG	418	49	10
		ASP	ACAGGACATTTCGTCA
HPI	IS100	SP	ATTGATCCACCGTTTTACTC	963	55	32
		ASP	CGAACGAAAGCATGAAACAA
HPI	psn	SP	CTTTCCACCAACACCATCC	1,062	55	6
		ASP	AAACCGCCACTTCGCTTC
HPI	yptE	SP	CCCTTACCCATTGCCGAAC	1,198	55	16
		ASP	TCCCCACCTCATCCAGCC
HPI	irp1	SP	AGAAACCGATGCTCACCC	526	55	16
		ASP	TCCTCTCCTGACGTAGCC
HPI	irp2	SP	AAGGATTCGCTGTTACCGGAC	280	55	39
		ASP	TCGTCGGGCAGCGTTTCTTCT
HPI	ybtP-ybtQ	SP	GCCGGGAACGTCAAAGAA	1,816	55	3
		ASP	AGGTGAGCTTTCATGTGCCT
HPI	ybtX-ybtS	SP	TCAGTCGAATGTGAAACCGC	1,453	55	3
		ASP	GCAGCCGTGCCTGGCACCCTTT
HPI	int	SP	TGCGCCATGCGGTCCATC	714	55	3
		ASP	GGTGCATAAGATTCTCGG
HPI	asnT-int	SP	ATCGCTTTGCGGGCTTCTAGGT	1,396	55	3
		ASP	GAACGGCGGACTGTTAAT

^aSP, sense primer; ASP, antisense primer.