Figure 2.

A comparison of different TRV₂-PDS vectors inoculated using vacuum infiltration. (a) The detection of the viral coat protein (CP) transcripts by RT-PCR in the cannabis line MF-219 infiltrated with pTRV₂-PDS₃₁₀ or pTRV2, and on pTRV₂ plasmid as a positive control. UBQ5 was used as a control gene. Cannabis plantlets inoculated with pTRV₁- and pTRV₂-PDS₃₁₀ (b), pTRV2-PDS₄₈₆ (c), pTRV₂-PDS₄₂₄ (d), or pTRV₂ (e) 15 days after vacuum infiltration (scale bar = 2 cm). (f) A quantitative real-time PCR analysis of PDS transcript levels in cannabis leaves infected with TRV₂ and TRV₂-PDS₃₁₀. The data were normalized to UBQ5 with the standard error indicated by vertical lines. The significant difference between treatments (p ≤ 0.0006; n ≥ 3; ***) was calculated using Student’s t test.