Table 4.
The maximal amounts of estimated metabolites in agitated microshoot in vitro cultures of S. lateriflora grown on LS medium supplemented with 1.0 mg/L BA and 1.0 NAA mg/L after administering different concentrations of the biosynthetic precursors phenylalanine (Phe) and tyrosine (Tyr), and the elicitor methyl jasmonate (MeJa), collected 7 days after supplementation.
| Metabolite [mg/100 g DW] |
7th Day | |||||
|---|---|---|---|---|---|---|
| Control | Phe | Tyr | MeJa | Phe + MeJa | Tyr + MeJa | |
| Baicalein | 162.414 ± 26.104 | 614.788 ± 38.279 b | 162.457 ± 49.878 b | 300.486 ± 40.901 e | 541.608 ± 36.215 b | 264.457 ± 31.590 a |
| Baicalin | 378.595 ± 26.606 | 988.086 ± 56.083 c | 419.209 ± 21.780 a | 474.399 ± 49.675 d | 630.484 ± 17.532 b | 411.619 ± 41.777 a |
| Wogonin | 666.088 ± 71.518 | 964.501 ± 49.668 b | 655.544 ± 30.779 a | 676.857 ± 55.040 e | 782.986 ± 29.100 b | 599.387 ± 51.315 a |
| Wogonoside | 469.987 ± 59.996 | 1148.771 ± 50.697 c | 516.879 ± 56.240 a | 365.481 ± 17.298 e | 620.747 ± 30.154 b | 479.433 ± 51.315 a |
| Scutellarin | 2.539 ± 0.940 | 68.295 ± 4.980 b | 9.321 ± 1.797 a | 10.087 ± 2.591 e | 18.946 ± 3.460 a | 10.042 ± 1.025 b |
| Oroxylin A | 0.214 ± 0.023 | 53.653 ± 4.450 c | 41.758 ± 8.250 b | 43.708 ± 3.768 d | 25.006 ± 7.145 c | 14.562 ± 2.246 a |
| Total flavonoids | 1679.837 ± 185.187 | 3764.881 ± 217.489 b | 1799.470 ± 137.499 a | 1814.878 ± 137.456 e | 2616.874 ± 120.568 b | 1778.499 ±179.168 a |
| Verbascoside | 267.327 ± 15.187 | 474.795 ± 27.489 b | 263.961 ± 37.499 a | 121.553 ± 19.489 d | 170.694 ± 17.156 a | 44.068 ± 4.892 b |
a 1.0 g/L; b 1.5 g/L; c 2.0 g/L; d 10 µM; e 50 µM.