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. 2022 Feb 3;27(3):1049. doi: 10.3390/molecules27031049

Figure 1.

Figure 1

Evaluation of coffee-derived circulating metabolite antioxidant properties against TBHP in RBE4 cells. (A) RBE4 cells were treated with 100 nM of coffee-derived circulating metabolites for 48 h or treated with tert-Butyl hydroperoxide (TBHP) 200 μM for three hours; then, the MTT assay was performed to evaluate cell viability. Dimethyl sulfoxide (DMSO) (as a solvent to dissolve Metabolites) was also evaluated. (B) Reactive oxygen species (ROS) intracellular evaluation by 2,7-dichlorofluorescein (DCF) fluorescence intensity of cells treated with TBHP 200 μM for three hours alone or in combination with 100 nM metabolite mix pretreatment. The histograms, obtained from three distinct experiments, are expressed as a percentage of cell viability or intracellular ROS ± S.E. *** p < 0.001 versus CTRL (untreated cells); ᐃᐃᐃ p < 0.001 versus TBHP (positive control).