Figure 3.

Evaluation of coffee pulp phytoextract antioxidant properties against TBHP in RBE4 cells. (A) RBE4 cells were treated with different concentrations (100, 300, 500 µg/mL) of phytoextract (Phyt) for 48 h. Then the MTT assay was performed to evaluate cell viability. (B) Intracellular ROS evaluation by DCF fluorescence intensity in RBE4 treated with TBHP 200 μM for three hours alone or in combination with 100 μg/mL pretreatment of Phyt. Phyt treatment was also tested. The histograms, obtained from three distinct experiments, are expressed as a percentage of cell viability or intracellular ROS as mean ± S.E. ** p <0.01, *** p < 0.001 versus CTRL (untreated cells); ᐃ p < 0.05 compared to TBHP.