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. 2022 Feb 3;27(3):1049. doi: 10.3390/molecules27031049

Figure 5.

Figure 5

Effect of coffee-pulp phytoextract on Akt and Erk1/2 activation. Cells pretreated or not for 24 h of 100 μg/mL Phyt and then subjected to OGD/ogR exposure were harvested in a lysis buffer. Equal amounts of homogenate samples (as protein) were analyzed by SDS-PAGE electrophoresis and Western blotting. (A) p-Akt, akt and (B) p-Erk1/2, Erk1/2 were detected with specific antibodies and revealed by enhanced chemiluminescence (ECL). Samples were normalized on β-actin immunoreactivity. Histograms, obtained from three distinct experiments, represent the percentage of protein levels with respect to control as mean ± S.E. * p < 0.05, ** p < 0.01, *** p < 0.001 versus CTRL (untreated cells); §§ p < 0.01, §§§ p < 0.001 versus ogR0h; # p < 0.05 versus ogR1h.