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. 2022 Feb 3;27(3):1049. doi: 10.3390/molecules27031049

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of coffee pulp phytoextract on HO-1 protein levels. Cells pretreated or not for 24 h with 100 μg/mL Phyt and then subjected to OGD/ogR exposure were harvested in a lysis buffer. Equal amounts of homogenate samples (as protein) were analyzed by SDS-PAGE electrophoresis and Western blotting. HO-1 was detected with a specific antibody and revealed by enhanced chemiluminescence (ECL). Samples were normalized on β-actin immunoreactivity. Histograms, obtained from three distinct experiments, represent the percentage of protein levels with respect to control as mean ± S.E. ** p < 0.01, *** p < 0.001 versus CTRL; §§ p < 0.01 versus ogR0h; ## p < 0.01 versus ogR1h.