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. 2022 Jan 20;14(3):451. doi: 10.3390/nu14030451

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) Results of cell density analysis in the cellular CA2 subregion of the hippocampus on postnatal day PND 12 (C, n = 5; LP, n = 7; LIG, n = 6; IUS, n = 7). For each animal, grayscale measurements were performed at three replicates per hippocampal region. All data were normalized to the mean of the control group (C). Representative images of cell density measurement of (b) control (C) offspring and (c) offspring after intrauterine stress (IUS) in the cellular CA2 subregion of the hippocampus on postnatal day PND 12 (HE staining, 20-fold magnification). White circles indicate regions of interest (ROIs) used for quantitative analysis. Scale bar = 50 μm. Asterisks indicate significance; *, adjusted p < 0.05.