Figure 5.

Western blot analyses of phospho-p70 S6 Kinase (p-p70S6K, Thr389) and p70S6K proteins in the hippocampus of male rats on postnatal days PND 1, PND 12 (C, n = 5; LP, n = 5; LIG, n = 5; IUS, n = 5) and PND 180 (C, n = 5; LP, n = 4; LIG, n = 5; IUS, n = 5). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as additional reference protein for quality assurance but not used for calculations. Densitometric ratios were calculated for p-p70S6K/p70S6K and are shown for each group (C, controls; LP, low protein; LIG, ligation; IUS, intrauterine stress) as mean ± SD directly below the appropriate Western blot signals. Data were compared by nonparametric one-way ANOVA. Dunn’s post-test was performed for the comparisons LP—C, LIG—C, IUS—C. There were no significant differences between the groups.