Table 1.
Primary antibodies.
| Antibody | Manufacturer | Molecular Weight | Dilution * |
|---|---|---|---|
| 4E-BP1 Rabbit mAb | Cell Signaling Technology (#9644) | 15–20 kDa | 1:1000 |
| Phospho-4E-BP1 (Thr37/46) Rabbit mAb | Cell Signaling Technology (#2855) | 15–20 kDa | 1:2000 |
| GAPDH Rabbit mAb | Cell Signaling Technology (#2118) | 20 kDa | 1:3000 |
| mTOR Rabbit mAb | Cell Signaling Technology (#2983) | 289 kDa | 1:1000 |
| Phospho-mTOR (Ser2448) Rabbit mAb | Cell Signaling Technology (#5536) | 289 kDa | 1:500 |
| p70S6K Rabbit mAb | Cell Signaling Technology (#2708) | 70/89 kDa | 1:500 |
| Phospho-p70S6K (Thr389) Rabbit mAb | Cell Signaling Technology (#9234) | 70/89 kDa | 1:500 |
| Tau Rabbit mAb | Abcam (ab32057) | 50–79 kDa | 1:2000 |
| Phospho-Tau (Ser396) Rabbit mAb | Abcam (ab109390) | 50–79 kDa | 1:10,000 |
* in 5% BSA/TBST.