Extended Data Fig. 1 |. Differential effects of tumour Ddr1-KO on tumour growth in vitro and in vivo.

(a) Immunoblotting of DDR1, DDR2 and loading control β-ACTIN in M-Wnt and AT-3 Ddr1-WT/KO tumour cells. Images are representatives of three independent experiments. (b–d) In vitro cell proliferation of E0771 (WT: n = 3, KO: n = 5, b), M-Wnt (WT: n = 3, KO: n = 5, c) and AT-3 (WT: n = 6, KO: n = 4, d) tumour cells, n indicate technical repeats. Out of three biological repeats. (e–f) M-Wnt (n = 4 tumours/group, e) and AT-3 (n = 5 tumours/group, f) tumour growth in immunodeficient mice. (g–h) M-Wnt (n = 7 tumours/group, g) and AT-3 (n = 7 tumours/group, h) tumour growth in immunocompetent C57BL/6 mice. (i–j) M-Wnt and AT-3 tumours were grown firstly in Rag1^−/− hosts. Approximately 60 mg of tumour pieces were transplanted to C57BL/6 mice. Tumour volume of M-Wnt (WT” n = 9 tumours, KO: n = 10 tumours, i) and AT-3 (WT: n = 10 tumours, KO: n = 9 tumours, j). (k) Percentage of CD8⁺ in CD3⁺ T cells in blood, n = 5 mice/group. (l) Tumour volumes in C57BL/6 hosts with prior treatment of anti-IgG or anti-CD8 antibody (n = 5 tumours/group). (m) CD8⁺ TILs normalized by tumour weight in Rag1^−/− mice after adoptive transfer of CD8⁺ T cells or medium (sham), n = 6 tumours/group. (n) Tumour volumes in Rag1^−/− mice after adoptive transfer of CD8⁺ T cells or medium (sham). n = 6 tumours/group. Arrow indicates transfer of CD8⁺ T cells on day 17. (o) Tumour weight from rechallenged mice (n = 6 tumours/group). Values represent mean ± SEM. p value and n as indicated, all tests used two-way ANOVA except for CD8⁺ quantification, which used two-tailed Student’s t-test.