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. 2022 Jan 29;14(3):604. doi: 10.3390/nu14030604

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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MFG-E8 small interfering RNA (siRNA) and overexpression plasmid transfection of STC-1. (A) Fluorescence micrograph displays MFG-E8 siRNA and overexpression in STC-1 cells at 48 h after transfection (×200). (B) Quantitative RT-PCR analysis demonstrates MFG-E8 mRNA expression in STC-1 at 48 h after transfection. (C) Western blot analysis demonstrates MFG-E8 protein expression in STC-1 at 48 h after transfection. (D) Data were expressed as the expression ratio of MFG-E8/β-actin. ** p < 0.01 compared with NC group; ^## p < 0.01 compared with GFP group. CC group: normal control cells; NC group: negative control siRNA cells; MsiRNA group: STC-1 carrying siRNA against MFG-E8; GFP group: STC-1 carrying GFP; Mover group: STC-1 carrying both GFP and MFG-E8.