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. 2022 Jan 28;14(3):575. doi: 10.3390/nu14030575

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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URAT1-inhibitory activity of each subfraction from the ethyl acetate fraction of the ethanolic extract of rooibos leaves. (a) A preparative MPLC chromatogram for separating the ethyl acetate fraction. The chromatogram was recorded at 280 nm. Blue and red lines indicate the linear gradients of solvent B (ethyl acetate) and solvent C (methanol), respectively. (b) URAT1-inhibitory activity profile of each subfraction (20 ppm) obtained from the column chromatography; 1% dimethyl sulfoxide was used for the vehicle control. All data are expressed as % of the vehicle control and the mean ± S.E.M.; n = 9 (Ctrl, control), 5 (the others). #, fraction number; *, p < 0.05; **, p < 0.01 vs. control (Dunnett’s test).