Table 1.
Validation results of the recommended HPLC method for analysis of ALB, LEV ABA, and CLO.
| Parameters | ALB | LEV | ABA | CLO |
|---|---|---|---|---|
| Range in (µg/mL) | 15.15–93.75 | 25–150 | 30–150 | 11.7–140.63 |
| Slope (mAU*s mL/µg) | 32.252 | 23.239 | 4.174 | 18.312 |
| Intercept (mAU*s) | −9.008 | −26.128 | 2.028 | 4.568 |
| R2 | 0.99984 | 0.99980 | 0.99997 | 0.99990 |
| Accuracy (mean ± SD) |
101.06 ± 0.489 | 101.15 ± 0.579 | 99.61 ± 0.588 | 100.58 ± 0.723 |
| Precision (%RSD) Repeatability * RSD ≤ 2% Day to day precision ** |
0.47 | 0.33 | 0.35 | 1.25 |
| Pooled RSD ≤ 3% | 1.47 | 1.62 | 0.29 | 2.03 |
| LOD in (µg/mL) *** | 1.669 | 2.989 | 1.167 | 1.991 |
| LOQ in (µg/mL) *** | 5.056 | 9.057 | 3.537 | 6.033 |
* The intra-day precision (n = 6), average of one concentration repeated six times in the same day. ** The inter-day precision (n = 3), 3 replicates of a single sample were implemented on the first day, and then, on a second day, 3 replicates of freshly prepared samples were analyzed. The same analyst performed both tests. *** Limits for detection and quantitation were calculated via LOD = (SD of the response/slope) × 3; LOQ = (SD of the response/slope) × 10.