Figure 6.

Effect of ETL on NF-κB nuclear localization in LPS-stimulated RAW264.7 cells. Cells were pre-treated with ETL (25–100 μg/mL) for 1 h and then treated with LPS (1 μg/mL) for 30 min (the untreated control/LPS(-), the LPS-treated group/LPS(+)). The cytosolic and nuclear extracts were subjected to Western blot analysis using anti-NF-κB p65, anti-NF-κB p50, anti-β-actin (cytosolic fraction loading control), and anti-lamin B (nuclear fraction loading control) antibodies. Data are expressed as the mean ± SD of three replicates.