Figure 4.
Scanning electron microscopy images of 72 h biofilms on titanium discs coated with: (A,B) phosphate buffered saline (PBS); (C,D) undoped nanoparticles (NPs); (E,F) calcium NPs; (G,H) zinc NPs; (I,J) doxycycline NPs. A mature biofilm could be seen in the negative control group (A,B). In unloaded NPs, calcium NPs and zinc NPs (C–H), there were similar biofilm structures and development, with isolated areas of the discs free from biofilm growth. Doxycycline NPs was the only group without an observable biofilm on the coated titanium surface. Magnification: (A,C,E,G,I) 1000×; (B,D,F,H,J) 3000×.