Table 1.
The content of steroids in Calendula officinalis hairy root cultures after elicitation with ethylene.
| Compound | ETY Concentration (µmol/L) | ||
|---|---|---|---|
| 0 | 10 | 100 | |
| campesterol | 52.6 ± 1.6 a | 41.3 ± 7.3 a | 58.1 ± 3.0 a |
| cholesterol | 2.9 ± 0.4 a | 6.1 ± 0.9 b | 7.2 ± 0.5 b |
| isofucosterol | 6.7 ± 0.8 a | 3.3 ± 1.1 b | 7.8 ± 0.7 a |
| 24-methylenecycloartanol | 11.4 ± 2.5 a | 11.5 ± 1.9 a | 7.1 ± 0.7 b |
| sitosterol | 144.0 ± 25.4 a | 70.7 ± 3.1 b | 71.7 ± 7.9 b |
| sitostanol | 15.6 ± 2.9 a | 10.7 ± 1.4 a | 9.5 ± 0.8 a |
| stigmasterol | 460.3 ± 4.2 a | 398.9 ± 53.4 a | 581.8 ± 21.9 b |
| stigmast-7-en-3-ol | 8.1 ± 2.5 a | 3.0 ± 0.7 b | 2.8 ± 0.2 b |
| tremulone | 13.2 ± 3.3 a | 9.7 ± 2.4 a | 2.1 ± 0.6 b |
| Total | 714.7 | 555.2 | 748.1 |
Results are referenced to the hairy root dry weight and expressed as the means ± SE (standard error) of three independent samples. The means in a line which do not share a common letter are significantly different (p < 0.05).