Effects of Atractylodes macrocephala and Amomum villosum extracts (3:1) on adipocyte differentiation in 3T3-L1 preadipocytes. Confluent 3T3-L1 preadipocytes were differentiated into adipocytes in media supplemented with or without AME:AVE (3:1) for 6 days. Lipid accumulation was measured via Oil-Red O staining and absorbance. Data represent mean ± SD of three independent experiments. Scale bar = 500 μm. CON, only dimethyl sulfoxide (DMSO) treated no differentiation control; MDI, only 3-isobutyl-1-methylxanthine, dexamethasone, and insulin treated differentiation control. ## p < 0.01 vs. CON group. * p < 0.05, and ** p < 0.01 vs. MDI group.