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Scheme of the velocity profile in time for the inlet with the analyte and the inlet containing the washing buffer.
| Time (min) | Flow Velocity Target (m/s) |
Flow Velocity Wash (m/s) |
Description | |
|---|---|---|---|---|
| 0 | 10 | 1.7 × 10−3 | 0 | Analyte injection |
| 10 | 25 | 0 | 0 | Incubation |
| 25 | 40 | 0 | 1.7 × 10−3 | Washing |
