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. 2022 Jan 24;12(3):368. doi: 10.3390/nano12030368

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Nil Red fluorescence stained images of vehicle control, NLCs-KAA, NLCs-PUNI-KAA, PUNI and orlistat treated maturing adipocyte after 14 days. Images showing less lipid accumulation in NLCs-PUNI-KAA when compared to all other treatments. In Nile Red fluorescence staining, vehicle control showing high red fluorescence directly proportional to intracellular lipid accumulation level (yellow arrow head). However, in NLCs-PUNI-KAA treatment found with less red fluorescence confirmed low levels of intracellular lipid accumulation (white arrow head). 1.6 µg/dL dose of NLCs-PUNI-KAA treatment showing highest inhibition of lipid accumulation compared to NLCs-KAA or PUNI or orlistat.