Fig. 2.

The functional result of platelet activation was positive in our patient: the positive functional result of platelet activation was presented in serum without heparin, the percentage of platelets activated for both anti-CD61 and anti-CD62p conjugated antibodies was 19.19% to indicate that platelet activation was triggered (data were shown in the upper right quadrant of each plot). In the presence of patient serum plus heparin (0.1 IU/mL, 0.3 IU/mL, and 100 IU/mL), donor platelets show the reduced reactivity with 12.8%, 12.3% and 15.8% of activated platelets. The definition of positive flow cytometric assay was with percentage of activated platelets more than 11%. Abbreviation: CD61 (glycoprotein IIIa): a marker of platelet identification; CD62p (p-selectin): a marker of platelet activation; Adenosine diphosphate (ADP): a positive control of normal platelet activation.