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. 2022 Feb 12;22:76. doi: 10.1186/s12935-022-02499-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Low expression of ZHX2 was associated with poor prognosis in thyroid cancer. A ZHX2 expression was measured in human tissue samples using western blotting (WB). GAPDH was used as the internal control. Thyroid tumour tissues (T); adjacent non-tumour tissues (P). B ZHX2 mRNA levels were evaluated by RT–qPCR in human specimens. **p < 0.01, the p value was determined by Student’s t test. C Representative images of ZHX2 immunostaining in thyroid adjacent non-tumour and thyroid tumour tissues (Image magnifications of 100 × , bar: 100 μm). D Statistical analysis of ZHX2 expression in thyroid adjacent non-tumour and thyroid tumour tissues. Significant differences were determined by the chi-squared test. E Kaplan–Meier survival curves of thyroid cancer patients with ZHX2 expression were analysed by online software