Fig. 5.

ZHX2 restrains thyroid cancer cell migration via repression of S100A14 in vitro. A–F ZHX2-overexpressing or ZHX2-knockdown KMH-2 and BHP10-3 cells were transfected with S100A14 or siS100A14, respectively. Transwell assays (A, D), wound healing assays (B, E) and protein levels of EMT markers N-cadherin, E-cadherin and vimentin (F, G) were assessed in these cells. The migrated cells were photographed. Image magnifications: 200 ×  (A, D), 40 ×  (B, E). Bar: 100 μm (A, D), 500 μm (B, E). C RT-qPCR analysis for knockdown of S100A14 in BHP10-3 cells were shown. Data are the mean ± SD. One-way ANOVA. *p < 0.05, **p < 0.01, ns not significant