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Screening for ZjPSY interacting proteins with yeast two-hybrid assay. Interaction between ZjJ2 and ZjPSY. Yeast cells were spotted on higher stringency QDO/X/A agar plates by 10-fold serial dilutions. Blue clones were positive and white or absent clones were negative. pGBKT7-53 or pGADT7-T was used as the negative control, and pGBKT7-53 and pGADT7-T were co-transformed as positive control.
