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. 2022 Feb 2;27(3):1025. doi: 10.3390/molecules27031025

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) Chemical structures of betulonic acid. (B) Growth inhibitory effects of betulonic acid on AGS. (C) Growth inhibitory effects of betulonic acid on HGC-27. (D) Growth inhibitory effects of betulonic acid on GES-1 cells treated with various concentrations of betulonic acid (20, 40, 80 and 160 μM) for 24 h, 48 h and 72 h; cell viability was assessed by CCK-8 assay. (E) After the colony formation experiment, cells were fixed and stained with crystal violet solution, and then the results were photographed. (F) Statistics of colony formation experiment results of AGS in gastric cancer cells. (G) Statistics of colony formation experiment results from HGC-27 gastric cancer cells. * p < 0.05, ** p < 0.01 and *** p < 0.001.