TABLE 4.
Evaluation of clinical NTM strains submitted to our laboratory in the span of 25 months
| Group | Species or identification | n | Comment |
|---|---|---|---|
| Corresponding with 100% similarity with a type strain sequence | M. abscessus | 1 | |
| M. avium or M. paratuberculosis | 4 | Phenotypically M. avium | |
| M. branderi | 5 | ||
| M. chelonae | 1 | ||
| M. fortuitum | 6 | ||
| M. gilvum | 1 | ||
| M. goodii | 6 | ||
| M. gordonae | 1 | ||
| M. kansasii or M. gastri | 6 | ||
| M. heckeshornense | 1 | ||
| M. lentiflavum | 7 | ||
| M. malmoense | 2 | ||
| M. marinum | 3 | ||
| M. obuense | 1 | ||
| M. peregrinum | 2 | ||
| M. petroleophilum | 1 | ||
| M. scrofulaceum | 1 | ||
| M. simiae | 2 | ||
| M. thermoresistibile | 4 | ||
| M. triplex | 1 | ||
| M. triviale | 1 | ||
| M. vaccae | 2 | ||
| M. xenopi | 2 | ||
| Subtotal | 61 | ||
| Divergent, but 16S rDNA sequence closely resembling that of an established species | M. interjectum-like | 1, 1 | 5- and 3-base divergence from type strain |
| M. gordonae-like | 1, 1, 1 | 3-, 3- and 5-base divergence from type strain; one of them shows salmon pigmentation | |
| M. gordonae group II (20) | 2 | 2-base divergence from type strain | |
| M. gordonae group III (20) | 1 | 1-base divergence from type strain | |
| M. xenopi-like | 1 | 1-base divergent; 1 Y at 213 | |
| M. xenopi-like | 1 | 27 ambiguities, 4 base variationsa | |
| M. kansasii subspecies (33) | 2 | 8-base divergence from type strain | |
| M. paraffinicum-like | 1, 1 | 2- and 3-base divergence from “M. paraffinicum” ATCC 12670 | |
| M. chelonae chemovar niacinogenes | 2 | 3-base divergence from type strain of M. chelonae | |
| M. novocastrense | 2 | 2-base divergence from type strain; phenotypically identical to M. flavescens | |
| M. neoaurum-like | 1 | 1-base divergence from type strain. | |
| Subtotal | 19 | ||
| Sequence corresponding to uncharacterized strain in GenBank | MCRO 6 [X93032] | 4 | 6-base divergence from M. nonchromogenicum |
| MCRO 8 [X93034] | 2 | 19-base divergence from M. lentiflavum; MACb probe positive | |
| MCRO 17 [X93028] | 5 | 6-base divergence from M. elephantis; 16 from M. pulveris | |
| Subtotal | 11 | ||
| Unique sequence, closest to | M. lentiflavum | 1, 2 | Differs by 15 and 17 bases; phenotypically similar to M. lentiflavum |
| M. austroafricanum | 1 | Differs by 35 bases | |
| M. fortuitum | 10 | Differs by 22 bases | |
| M. terrae | 1, 1, 2, 1 | Differs by 11, 12, 27, and 28 bases | |
| M. heckeshornense | 2 | Differs by 37 bases | |
| M. monacense | 1 | Differs by 14 bases | |
| M. chelonae | 1 | Differs by 21 bases; grew only on iron uptake medium | |
| M. bohemicum | 2 | Differs by 9 bases | |
| M. malmoense | 1 | Differs by 9 bases | |
| M. goodii | 1 | Differs by 10 bases | |
| M. triviale | 1 | Differs by 43 bases | |
| M. wolinskyi | 2 | Differs by 20 bases | |
| M. agri | 1 | Differs by 10 bases | |
| Subtotal | 31 |
a
The ambiguities in this isolate were represented by double peaks which corresponded to each base of M. heckeshornense and M. xenopi where the two species differ. Please refer to the text.
b
MAC, M. avium complex.