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Specificity of 68Ga-FAPI-46 binding to SF-539, U87-MGvIII, and A549 cells (1 h, 4 °C), with or without pre-incubation with 100-fold molar excess of natGa-FAPI-46 (A). FAP expression in SF-539, U87-MGvIII, and A549 cell lines determined by flow cytometry (B). Internalization of 68Ga-FAPI-46 in SF-539 cells (C). Data are expressed as mean ± SEM (n = 3 independent experiments). Statistical significance is represented as ** p < 0.01 and *** p < 0.001, ns = not significant.
